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These beneficial effects on the vasculature were associated with attenuation of leukocyte recruitment, decreased expression of pro-inflammatory mediators, including the NFBsubunit p65, VCAM-1, and monocyte chemotactic protein 1 (MCP-1) and reduced oxidative stress [86]

doi: 10.1016/j.jbc.2022.102148 32 GaussmannS.GopalswamyM.EberhardtM.ReuterM.ZouP.SchliebsW

Subsequently, the sonicated supernatants and sediments were analyzed using sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) and stained with the Coomassie Blue Staining Kit (Sigma, USA)

3.1.2 Tumor necrosis factor receptor-associated factor 6 (TRAF6)/NLRP3 pathway TRAF6 is a vital binding protein of the TNF and the TLR superfamily and is notably involved in innate immunity and acquired immunity